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验证数据展示

  • WB analysis using 86979-1-RR (same clone as 86979-1-PBS)

    WB analysis using 86979-1-RR (same clone as 86979-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86979-1-RR (ID3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86979-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86979-1-RR (same clone as 86979-1-PBS)

    IF Staining of HeLa using 86979-1-RR (same clone as 86979-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ID3 antibody (86979-1-RR, Clone: 252047B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86979-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02842-1

    Sandwich ELISA sample test of MP02842-1

    The mean ID3 concentration was determined to be 55.78 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 17.49 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02842-1

    Sandwich ELISA standard curve of MP02842-1

    Sandwich ELISA standard curve of MP02842-1, Human ID3 Recombinant Matched Antibody Pair - PBS only. 86979-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0588. 86979-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • Affinity and Kinetic Characterization of 86979-1-RR

    Affinity and Kinetic Characterization of 86979-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86979-1-RR against Human ID3 were performed. The affinity constant is 0.184 nM.

ID3 Recombinant monoclonal antibody, PBS Only (Capture)

ID3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA
Cat No. 86979-1-PBS

产品说明书

CloneNo. 252047B8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

别名

1R21, bHLHb25, Class B basic helix-loop-helix protein 25, DNA-binding protein inhibitor ID-3, HEIR 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86979-1-PBS targets ID3 as part of a matched antibody pair:

MP02842-1: 86979-1-PBS capture and 86979-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0588

Product name: Recombinant human ID3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-119 aa of BC003107

Sequence: MKALSPVRGCYEAVCCLSERSLAIARGRGKGPAAEEPLSLLDDMNHCYSRLRELVPGVPRGTQLSQVEILQRVIDYILDLQVVLAEPAPGPPDGPHLPIQTAELAPELVISNDKRSFCH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
别名 1R21, bHLHb25, Class B basic helix-loop-helix protein 25, DNA-binding protein inhibitor ID-3, HEIR 1
计算分子量119 aa, 13 kDa
观测分子量 13-15 kDa
GenBank蛋白编号BC003107
基因名称 ID3
Gene ID (NCBI) 3399
RRIDAB_3745251
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ02535
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The helix-loop-helix (HLH) family of transcription factors plays a central role in the regulation of cell growth, differentiation and tumourigenesis. Members of the Id (inhibitor of DNA binding) class of these nuclear proteins are able to heterodimerise with and thereby antagonise the functions of other transcription factors of this family. Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein (ID3, synonym: HEIR-1) is a member of the ID family of HLH proteins, which lacks a basic DNA-binding domain and inhibits transcription through formation of nonfunctional dimers that are incapable of binding to DNA.

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