验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse heart tissue, HepG2 cells, rat heart tissue, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86938-1-RR targets COX5A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2005 Product name: Recombinant human COX5A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC024240 Sequence: MLGAALRRCAVAATTRADPRGLLHSARTPGPAVAIQSVRCYSHGSQETDEEFDARWVTYFNKPDIDAWELRKGINTLVTYDMVPEPKIIDAALRACRRLNDFASTVRILEVVKDKAGPHKEIYPYVIQELRPTLNELGISTPEELGLDKV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | cytochrome c oxidase subunit Va |
| 别名 | COX VA, COX5, COXVA |
| 计算分子量 | 150 aa, 17 kDa |
| 观测分子量 | 13 kDa |
| GenBank蛋白编号 | BC024240 |
| 基因名称 | COX5A |
| Gene ID (NCBI) | 9377 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P20674 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COX5A(Cytochrome c oxidase subunit 5A, mitochondrial)is also named COXVA and belongs to the cytochrome c oxidase subunit 5A family. This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. The mature human COX Va polypeptide shares 35% and 95% protein sequence identity with the corresponding S. cerevisiae COX subunit VI and bovine COX Va, respectively(PMID:2853101).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for COX5A antibody 86938-1-RR | Download protocol |
| IHC protocol for COX5A antibody 86938-1-RR | Download protocol |
| WB protocol for COX5A antibody 86938-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
