验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293T cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86902-1-RR targets GLIS1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag40019 Product name: Recombinant human GLIS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 360-620 aa of BC101799 Sequence: GPDTEADVLTECLVLQQLHTSTQLAASDGKGGCGLGQELLPGVYPGSITPHNGLASGLLPPAHDVPSRHHPLDATTSSHHHLSPLPMAESTRDGLGPGLLSPIVSPLKGLGPPPLPPSSQSHSPGGQPFPTLPSKPSYPPFQSPPPPPLPSPQGYQGSFHSIQSCFPYGDCYRMAEPAAGGDGLVGETHGFNPLRPNGYHSLSTPLPATGYEALAEASCPTALPQQPSEDVVSSGPEDCGFFPNGAFDHCLGHIPSIYTDT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | GLIS family zinc finger 1 |
| 别名 | GLI similar 1, GLIS family zinc finger 1, GLI-similar 1, Zinc finger protein GLIS1 |
| 计算分子量 | 620 aa, 66 kDa |
| 观测分子量 | 66 kDa |
| GenBank蛋白编号 | BC101799 |
| 基因名称 | GLIS1 |
| Gene ID (NCBI) | 148979 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8NBF1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GLIS1 is a nuclear zinc finger transcription factor that integrates developmental signaling and transcriptional control during neural and epithelial differentiation. It enhances cellular reprogramming efficiency and modulates pathways linked to Hedgehog and Wnt signaling. Dysregulated GLIS1 expression contributes to cardiac malformations, cancer progression, and stemness reactivation, highlighting its dual role in developmental regulation and disease pathogenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GLIS1 antibody 86902-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
