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验证数据展示

  • WB analysis using 86883-1-RR (same clone as 86883-1-PBS)

    WB analysis using 86883-1-RR (same clone as 86883-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86883-1-RR (CCDC28A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86883-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 86883-1-RR (same clone as 86883-1-PBS)

    IF Staining of MCF-7 using 86883-1-RR (same clone as 86883-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CCDC28A antibody (86883-1-RR, Clone: 251922E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86883-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 86883-1-RR (same clone as 86883-1-PBS)

    IHC staining of mouse testis using 86883-1-RR (same clone as 86883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86883-1-RR (CCDC28A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86883-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02764-1

    Cytometric bead array sample test of MP02764-1

    The mean CCDC28A concentration was determined to be 70.2 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02764-1

    Cytometric bead array standard curve of MP02764-1

    Cytometric bead array standard curve of MP02764-1, CCDC28A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86883-2-PBS. Detection antibody: 86883-1-PBS. Standard: Ag6529. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 86883-1-RR

    Affinity and Kinetic Characterization of 86883-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86883-1-RR against Human CCDC28A were performed. The affinity constant is below 1 pM.

CCDC28A Recombinant monoclonal antibody, PBS Only (Detector)

CCDC28A Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86883-1-PBS

产品说明书

CloneNo. 251922E2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

C6orf80, CC28A, CCRL1AP, Coiled-coil domain-containing protein 28A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86883-1-PBS targets CCDC28A as part of a matched antibody pair:

MP02764-1: 86883-2-PBS capture and 86883-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6529

Product name: Recombinant human CCDC28A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-162 aa of BC000758

Sequence: MPKKNAIPVSKSTGFSNPASQSTSQRPKLKRVMKEKTKPQGGEGKGAQSTPIQHSFLTDVSDVQEMERGLLSLLNDFHSGKLQAFGNECSIEQMEHVRGMQEKLARLNLELYGELEELPEDKRKTASDSNLDRLLSDLEELNSSIQKLHLADAQDVPNTSAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 coiled-coil domain containing 28A
别名 C6orf80, CC28A, CCRL1AP, Coiled-coil domain-containing protein 28A
计算分子量 30 kDa
观测分子量25 kDa
GenBank蛋白编号BC000758
基因名称 CCDC28A
Gene ID (NCBI) 25901
RRIDAB_3745184
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IWP9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Coiled-coil domain-containing protein 28A (CCDC28A) is a translocation partner of nucleoporin 98 (NUP98) in acute leukemias, resulting in the creation of fusion genes and the expression of chimeric proteins. These fusion proteins, such as NUP98-CCDC28A, have been shown to promote myeloproliferative neoplasms (PMID: 22058212). Additionally, CCDC28A is essential for head-tail coupling stability, thereby ensuring sperm motility (PMID: 39500989).

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