验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, K-562 cells, Raji cells, SKOV-3 cells, COLO 205 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86853-1-RR targets ABCB10 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag6203 Product name: Recombinant human ABCB10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 452-738 aa of BC064930 Sequence: YSELMKGLGAGGRLWELLEREPKLPFNEGVILNEKSFQGALEFKNVHFAYPARPEVPIFQDFSLSIPSGSVTALVGPSGSGKSTVLSLLLRLYDPASGTISLDGHDIRQLNPVWLRSKIGTVSQEPILFSCSIAENIAYGADDPSSVTAEEIQRVAEVANAVAFIRNFPQGFNTVVGEKGVLLSGGQKQRIAIARALLKNPKILLLDEATSALDAENEYLVQEALDRLMDGRTVLVIAHRLSTIKNANMVAVLDQGKITEYGKHEELLSKPNGIYRKLMNKQSFISA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ATP-binding cassette, sub-family B (MDR/TAP), member 10 |
| 别名 | ABC transporter 10 protein, ABC-me protein, ABC-mitochondrial erythroid protein, ATP-binding cassette sub-family B member 10, mitochondrial, ATP-binding cassette transporter 10 |
| 计算分子量 | 79 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC064930 |
| 基因名称 | ABCB10 |
| Gene ID (NCBI) | 23456 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NRK6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ABCB10 (also known as ABC-me or M-ABC2) is an inner-mitochondrial-membrane ATP-binding cassette transporter that forms homodimers with its nucleotide-binding domain facing the matrix. ABCB10 is now recognized as a multifunctional protein that couples mitochondrial export activity to cellular antioxidant defence. During red-cell development it stabilises the iron importer mitoferrin-1 (Mfrn1), thereby sustaining heme synthesis, whereas in cardiac and other non-erythroid tissues it attenuates oxidative damage by limiting mitochondrial ROS accumulation. PMID: 22884976
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ABCB10 antibody 86853-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
