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验证数据展示

  • WB analysis using 86852-2-RR

    WB analysis using 86852-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86852-2-RR (FNBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 86852-2-RR

    IF Staining of Jurkat using 86852-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using FBP17 antibody (86852-2-RR, Clone: 251876G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

FBP17 Recombinant monoclonal antibody

FBP17 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86852-2-RR

产品说明书

CloneNo. 251876G12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

FNBP1, Formin-binding protein 1, Formin-binding protein 17

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, U2OS cells, MG-63 cells, Raji cells, MKN-45 cells, 143B cells
Positive IF/ICC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy FBP17 Ready-To-Use IHC Kit. FBP17 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86852-2-RR targets FBP17 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag25784

Product name: Recombinant human FNBP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 176-241 aa of BC101755

Sequence: AQIRHQMAEDSKADYSSILQKFNHEQHEYYHTHIPNIFQKIQEMEERRIVRMGESMKTYAEVDRQV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 formin binding protein 1
别名 FNBP1, Formin-binding protein 1, Formin-binding protein 17
观测分子量 80-85 kDa
GenBank蛋白编号BC101755
基因名称 FBP17
Gene ID (NCBI) 23048
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96RU3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBP17 (formin-binding protein 17) is a new dynamin-associating molecule consisting of several functional domains, including an N-terminal EFC domain and an SH3 domain at the C-terminus. FBP17 is involved in dynamin-mediated endocytosis and has been reported to be required for invadopodia formation and tumor cell invasion.

实验方案

Product Specific Protocols
IF protocol for FBP17 antibody 86852-2-RRDownload protocol
WB protocol for FBP17 antibody 86852-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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