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验证数据展示

  • WB analysis using 86827-4-RR

    WB analysis using 86827-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86827-4-RR (ANKRD46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86827-4-RR

    WB analysis using 86827-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86827-4-RR (ANKRD46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 86827-4-RR

    IF Staining of HEK-293 using 86827-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ANKRD46 antibody (86827-4-RR, Clone: 251840D6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of Jurkat using 86827-4-RR

    IF Staining of Jurkat using 86827-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ANKRD46 antibody (86827-4-RR, Clone: 251840D6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86827-4-RR

    Affinity and Kinetic Characterization of 86827-4-RR

    Biolayer interferometry (BLl) kinetic assays of 86827-4-RR against Human ANKRD46 were performed. The affinity constant is 0.351 nM.

ANKRD46 Recombinant monoclonal antibody

ANKRD46 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86827-4-RR

产品说明书

CloneNo. 251840D6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

ANK-S, ankyrin repeat domain 46, Ankyrin repeat domain-containing protein 46

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, MDA-MB-231 cells，HepG2 cells, Jurkat cells, rat brain tissue
Positive IF/ICC detected inJurkat cells, HEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86827-4-RR targets ANKRD46 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26623

Product name: Recombinant human ANKRD46 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-189 aa of BC060843

Sequence: MSYVFVNDSSQTNVPLLQACIDGDFNYSKRLLESGFDPNIRDSRGRTGLHLAAARGNVDICQLLHKFGADLLATDYQGNTALHLCGHVDTIQFLVSNGLKIDICNHQGATPLVLAKRRGVNKDVIRLLESLEEQEVKGFNRGTHSKLETMQTAESESAMESHSLLNPNLQQGEGVLSSFRTTWQEFVED

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ankyrin repeat domain 46
别名 ANK-S, ankyrin repeat domain 46, Ankyrin repeat domain-containing protein 46
观测分子量 25 kDa
GenBank蛋白编号BC060843
基因名称 ANKRD46
Gene ID (NCBI) 157567
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ86W74
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANKRD46 encodes a protein containing multiple ankyrin repeats. Ankyrin domains function in protein-protein interactions in a variety of cellular processes. ANKRD46 also known as ankyrin repeat small protein (ANK-S), is a 228-amino acid single-pass membrane protein(PMID: 21219636).

实验方案

Product Specific Protocols
IF protocol for ANKRD46 antibody 86827-4-RRDownload protocol
WB protocol for ANKRD46 antibody 86827-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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