验证数据展示
经过测试的应用
| Positive WB detected in | TGF beta 1 treated A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86773-2-RR targets Phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | SMAD family member 3 |
| 别名 | p SMAD3 (Ser423/425), Phospho SMAD3, Phospho SMAD3 (Ser423/425), Phospho-SMAD3, Phospho-SMAD3 (Ser423/425) |
| 计算分子量 | 48 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC050743 |
| 基因名称 | SMAD3 |
| Gene ID (NCBI) | 4088 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P84022 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. Phospho-SMAD3 (Ser423/425) is the dual-phosphorylated, active form of SMAD3 that results from TGF-β, activin or BMP receptor kinase signaling. Upon ligand binding, the type II receptor phosphorylates the type I receptor, which in turn phosphorylates SMAD3 at Ser423 and Ser425 within the SSXS motif. This creates a binding surface for SMAD4, forming the heterotrimeric transcription complex that translocates to the nucleus to regulate genes involved in cell cycle control, fibrosis, immune modulation and epithelial-mesenchymal transition (EMT).
