验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, HSC-T6 cells, K-562 cells, NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86693-2-RR targets NQO1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag2009 Product name: Recombinant human NQO1 protein Source: e coli.-derived, PKG Tag: GST Domain: 1-274 aa of BC007659 Sequence: MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | NAD(P)H dehydrogenase, quinone 1 |
| 别名 | Azoreductase, DHQU, DIA 4, DIA4, DT diaphorase |
| 计算分子量 | 274 aa, 31 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | BC007659 |
| 基因名称 | NQO1 |
| Gene ID (NCBI) | 1728 |
| RRID | AB_3745057 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P15559 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640, PMID:18848868)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NQO1 antibody 86693-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Procyanidin Capsules Combat ALF by Restoring Mitochondrial Homeostasis and Inhibiting Necroptosis via the PGAM5/DRP1/PINK1 Pathway. | ||
Phytomedicine Farrerol mitigates fungal keratitis by enhancing fungal clearance and limiting inflammation through autophagy and Nrf2 pathway. | ||
Phytomedicine Cannabidiol attenuates methamphetamine-induced psychosis via anti-oxidative stress: σ1R-mediated mitochondrial dysfunction as a critical pathway. | ||
Int J Biol Macromol The gut-liver axis mediates the protective effect of Cinnamomum chago polysaccharide against acute liver injury: Insights from integrated microbiome and metabolomics analysis. | ||
Int J Biol Macromol Effects of different deproteinization methods on the hepatoprotective activity of Smilax china L. polysaccharides against CCl(4)-induced acute liver injury in mice. | ||
Antioxidants (Basel) Propyl Gallate Attenuates Cognitive Deficits Induced by Chronic Sleep Deprivation Through Nrf2 Activation and NF-κB Inhibition. |
