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验证数据展示

  • WB analysis using 86651-1-RR (same clone as 86651-1-PBS)

    WB analysis using 86651-1-RR (same clone as 86651-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86651-1-RR (Nucleoside phosphorylase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86651-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02719-1

    Sandwich ELISA sample test of MP02719-1

    The mean Nucleoside phosphorylase concentration was determined to be 313.73 ng/mL in Jurkat cell extract based on a 1.20 mg/mL extract load.

  • Sandwich ELISA sample test of MP02719-1

    Sandwich ELISA sample test of MP02719-1

    Plasma of six individual healthy human donors was measured. The Nucleoside phosphorylase concentration of detected samples was determined to be 38.13 ng/mL with a range of 18.89-62.76 ng/mL.

  • Sandwich ELISA standard curve of MP02719-1

    Sandwich ELISA standard curve of MP02719-1

    Sandwich ELISA standard curve of MP02719-1, Human Nucleoside phosphorylase Recombinant Matched Antibody Pair - PBS only. 86651-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12507. 86651-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • IF Staining of NIH/3T3 using 86651-1-RR (same clone as 86651-1-PBS)

    IF Staining of NIH/3T3 using 86651-1-RR (same clone as 86651-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using NP antibody (86651-1-RR, Clone: 251603E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86651-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86651-1-RR

    Affinity and Kinetic Characterization of 86651-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86651-1-RR against Human Nucleoside phosphorylase were performed. The affinity constant is below 1 pM.

Nucleoside phosphorylase Recombinant monoclonal antibody, PBS Only (Detector)

Nucleoside phosphorylase Uni-rAb® Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA
Cat No. 86651-1-PBS

产品说明书

CloneNo. 251603E11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

别名

PNP, EC:2.4.2.1, Inosine phosphorylase, Inosine-guanosine phosphorylase, PRO1837

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86651-1-PBS targets Nucleoside phosphorylase as part of a matched antibody pair:

MP02719-1: 86651-2-PBS capture and 86651-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12507

Product name: Recombinant human NP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-289 aa of BC106074

Sequence: MENGYTYEDYKNTAEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYGEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANHEEVLAAGKQAAQKLEQFVSILMASIPLPDKAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nucleoside phosphorylase
别名 PNP, EC:2.4.2.1, Inosine phosphorylase, Inosine-guanosine phosphorylase, PRO1837
计算分子量 289 aa, 32 kDa
观测分子量32 kDa
GenBank蛋白编号BC106074
基因名称 Purine nucleoside phosphorylase
Gene ID (NCBI) 4860
RRIDAB_3745030
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP00491
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Purine nucleoside phosphorylases (PNP) is a ubiquitous enzyme of purine metabolism that functions in the salvage pathway, including even those of protozoan parasites, thus enabling the cells to utilize purine bases recovered from metabolized purine ribo- and deoxyribonucleosides to synthesize purine nucleotides (PMID: 11337031, 23332162). PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effects on B-cell function. hPNP is widely distributed in various tissues and cells of the human body, with the highest activity in kidney, peripheral lymphocytes and granulocytes (PMID: 38701712).

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