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验证数据展示

  • WB analysis using 86635-1-RR (same clone as 86635-1-PBS)

    WB analysis using 86635-1-RR (same clone as 86635-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86635-1-RR (SLC25A24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86635-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02724-1

    Sandwich ELISA sample test of MP02724-1

    The mean SLC25A24 concentration was determined to be 3,148.64 ng/mL in HEK-293 cell extract based on a 1.20 mg/mL extract load and 3,459.34 ng/mL in A431 cell extract based on a 1.20 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02724-1

    Sandwich ELISA standard curve of MP02724-1

    Sandwich ELISA standard curve of MP02724-1, Human SLC25A24 Recombinant Matched Antibody Pair - PBS only. 86635-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6334. 86635-1-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 ng/mL.

  • IHC staining of Human Hepatocellular cancer using 86635-1-RR (same clone as 86635-1-PBS)

    IHC staining of Human Hepatocellular cancer using 86635-1-RR (same clone as 86635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 86635-1-RR (SLC25A24 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86635-1-PBS in a different storage buffer formulation.

SLC25A24 Recombinant monoclonal antibody, PBS Only (Detector)

SLC25A24 Uni-rAb® Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA
Cat No. 86635-1-PBS

产品说明书

CloneNo. 251563C10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Sandwich ELISA, Indirect ELISA

别名

APC1, MCSC1, Mitochondrial adenyl nucleotide antiporter SLC25A24, Mitochondrial ATP-Mg/Pi carrier protein 1, Mitochondrial Ca(2+)-dependent solute carrier protein 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86635-1-PBS targets SLC25A24 as part of a matched antibody pair:

MP02724-1: 86635-2-PBS capture and 86635-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6334

Product name: Recombinant human SLC25A24 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 128-477 aa of BC068561

Sequence: ELILQSIDVDGTMTVDWNEWRDYFLFNPVTDIEEIIRFWKHSTGIDIGDSLTIPDEFTEDEKKSGQWWRQLLAGGIAGAVSRTSTAPLDRLKIMMQVHGSKSDKMNIFGGFRQMVKEGGIRSLWRGNGTNVIKIAPETAVKFWAYEQYKKLLTEEGQKIGTFERFISGSMAGATAQTFIYPMEVMKTRLAVGKTGQYSGIYDCAKKILKHEGLGAFYKGYVPNLLGIIPYAGIDLAVYELLKSYWLDNFAKDSVNPGVMVLLGCGALSSTCGQLASYPLALVRTRMQAQAMLEGSPQLNMVGLFRRIISKEGIPGLYRGITPNFMKVLPAVGISYVVYENMKQTLGVTQK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 24
别名 APC1, MCSC1, Mitochondrial adenyl nucleotide antiporter SLC25A24, Mitochondrial ATP-Mg/Pi carrier protein 1, Mitochondrial Ca(2+)-dependent solute carrier protein 1
计算分子量 53 kDa
观测分子量48 kDa
GenBank蛋白编号BC068561
基因名称 SLC25A24
Gene ID (NCBI) 29957
RRIDAB_3745021
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ6NUK1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC25A24 (solute carrier family 25 member 24) is a gene encoding a mitochondrial ATP-Mg/phosphate transporter. It belongs to the mitochondrial carrier protein family and is mainly involved in mitochondrial energy metabolism and calcium signal regulation.

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