验证数据展示
经过测试的应用
| Positive WB detected in | human skeletal muscle tissue, mouse skeletal muscle tissue, human heart tissue, mouse heart tissue, rat heart tissue, pig heart tissue |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy FABP3 Ready-To-Use IHC Kit. FABP3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86511-3-RR targets FABP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag1069 Product name: Recombinant human FABP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-133 aa of BC007021 Sequence: MVDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTFKNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLILTLTHGTAVCTRTYEKEA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor) |
| 别名 | Fatty acid binding protein 3, Fatty acid-binding protein 3, Fatty acid-binding protein, heart, Heart-type fatty acid-binding protein, H-FABP |
| 计算分子量 | 15 kDa |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | BC007021 |
| 基因名称 | FABP3 |
| Gene ID (NCBI) | 2170 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P05413 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FABP3 (fatty-acid-binding protein 3), also known as heart-type FABP or mammary-derived growth inhibitor (MDGI), is a small 15-kDa cytoplasmic protein transporting fatty acids and other lipophilic substances from the cytoplasm to the nucleus. It is most ubiquitously expressed in heart and skeletal muscle.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for FABP3 antibody 86511-3-RR | Download protocol |
| WB protocol for FABP3 antibody 86511-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
