验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, HEK-293 cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86488-1-RR targets SECISBP2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag3541 Product name: Recombinant human SECISBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 506-854 aa of BC036109 Sequence: NPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERKQRLQENAVGPAFTSDDTQDGESGGDDQFPEQAELSGPEGMDELISTPSVEDKSEEPPGTELQRDTEASHLAPNHTTFPKIHSRRFRDYCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLKKLKCVIISPNCEKIQSKGGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQDQFHKMVELTVAARQAYKTMLENVQQELVGEPRPQAPPSLPTQGPSCPAEDGPPALKEKEEPHYIEIWKKHLEAYSGCTLELEESLEASTSQMMNLNL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | SECIS binding protein 2 |
| 别名 | SBP2, SECIS binding protein 2, SECIS-binding protein 2, Selenocysteine insertion sequence-binding protein 2 |
| 计算分子量 | 854 aa, 95 kDa |
| 观测分子量 | 95 kDa |
| GenBank蛋白编号 | BC036109 |
| 基因名称 | SECISBP2 |
| Gene ID (NCBI) | 79048 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q96T21 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SECISBP2 (also called SECIS-binding protein 2 or SBP2) is an RNA-binding protein that serves as the master regulator of selenoprotein synthesis in eukaryotes. It recognizes and binds the SECIS (SEC Insertion Sequence) stem-loop structure located in the 3′-UTR of all selenoprotein mRNAs, thereby converting the otherwise stop-coding UGA triplet into the 21st amino acid selenocysteine (Sec) during translation.
