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验证数据展示

  • WB analysis using 86462-1-RR

    WB analysis using 86462-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86462-1-RR (BRSK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse stomach using 86462-1-RR

    IHC staining of mouse stomach using 86462-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 86462-1-RR (BRSK1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat stomach using 86462-1-RR

    IHC staining of rat stomach using 86462-1-RR

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 86462-1-RR (BRSK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat stomach using 86462-1-RR

    IHC staining of rat stomach using 86462-1-RR

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 86462-1-RR (BRSK1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 86462-1-RR

    Affinity and Kinetic Characterization of 86462-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86462-1-RR against Human BRSK1 were performed. The affinity constant is 0.173 nM.

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BRSK1 Recombinant monoclonal antibody

BRSK1 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 86462-1-RR

产品说明书

CloneNo. 251341A11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

BR serine/threonine kinase 1, BR serine/threonine-protein kinase 1, Brain-selective kinase 1, Brain-specific serine/threonine-protein kinase 1, EC:2.7.11.1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inrat stomach tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86462-1-RR targets BRSK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3357

Product name: Recombinant human BRSK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 475-778 aa of BC016681

Sequence: TQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSGGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGAPPRSLQPPPGRPDPELSSSPRRAPPKDKKLLATNGTPLP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BR serine/threonine kinase 1
别名 BR serine/threonine kinase 1, BR serine/threonine-protein kinase 1, Brain-selective kinase 1, Brain-specific serine/threonine-protein kinase 1, EC:2.7.11.1
计算分子量 87 kDa
观测分子量 85-90 kDa
GenBank蛋白编号BC016681
基因名称 BRSK1
Gene ID (NCBI) 84446
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8TDC3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRSK1(Brain-specific serine/threonine-protein kinase 1) is also named as KIAA1811, SAD1, SADB and belongs to the SNF1 subfamily. BRSKs are crucial for establishing neuronal polarity, and BRSK1 has also been shown to regulate neurotransmitter release presynaptically. BRSK1 is a serine-threonine kinases specifically expressed in the brain, and activated by LKB1-mediated phosphorylation of a threonine residue at their T-loop. (PMID:22020259). It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for BRSK1 antibody 86462-1-RRDownload protocol
IHC protocol for BRSK1 antibody 86462-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols