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验证数据展示

  • IHC staining of human tonsil using 86457-4-RR (same clone as 86457-4-PBS)

    IHC staining of human tonsil using 86457-4-RR (same clone as 86457-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CK8 antibody included
  • IHC staining of human tonsil using 86457-4-RR (same clone as 86457-4-PBS)

    IHC staining of human tonsil using 86457-4-RR (same clone as 86457-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CK8 antibody included
  • IF Staining of A431 using 86457-4-RR (same clone as 86457-4-PBS)

    IF Staining of A431 using 86457-4-RR (same clone as 86457-4-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 8 antibody (86457-4-RR, Clone: 251045E11 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

  • WB analysis using 86457-4-RR (same clone as 86457-4-PBS)

    WB analysis using 86457-4-RR (same clone as 86457-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02886-1

    Sandwich ELISA sample test of MP02886-1

    The mean Cytokeratin 8 concentration was determined to be 116.99 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 15.41 ng/mL in HepG2 cell extract based on a 1.20 mg/mL extract load and 2.54 ng/mL in SH-SY5Y cell extract based on a 1.20 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02886-1

    Sandwich ELISA standard curve of MP02886-1

    Sandwich ELISA standard curve of MP02886-1, Human Cytokeratin 8 Recombinant Matched Antibody Pair - PBS only. 86457-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY02545. 86457-8-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • IF Staining of human breast cancer using 86457-4-RR (same clone as 86457-4-PBS)

    IF Staining of human breast cancer using 86457-4-RR (same clone as 86457-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 8 antibody (86457-4-RR, Clone: 251045E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

Cytokeratin 8 Recombinant monoclonal antibody, PBS Only (Capture)

Cytokeratin 8 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA
Cat No. 86457-4-PBS

产品说明书

CloneNo. 251045E11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA

别名

KRT8, CARD2, CK 8, CK8, CK-8

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CK8 Ready-To-Use IHC Kit. CK8 primary antibody included.

产品信息

86457-4-PBS targets Cytokeratin 8 as part of a matched antibody pair:

MP02886-1: 86457-4-PBS capture and 86457-8-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratin 8
别名 KRT8, CARD2, CK 8, CK8, CK-8
计算分子量 54 kDa
观测分子量52 kDa
GenBank蛋白编号BC000654
基因名称 Cytokeratin 8
Gene ID (NCBI) 3856
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05787
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytokeratin 8 (CK8) is a protein that belongs to the cytokeratin family, which is a group of structural proteins found in the intracytoplasmic cytoskeleton of epithelial cells. CK8 forms intermediate filaments within the cytoplasm of simple epithelial cells, typically as a dimer with cytokeratin 18 (CK18). These filaments provide a stabilizing framework that helps determine cell shape and enables cells to cope with mechanical stress. CK8/CK18 filaments also serve as a mesh of "paths" for signaling molecules, metabolites, and pathogens to travel within the cell in an organized manner. CK8 is expressed in various epithelial tissues and is particularly associated with glandular and simple epithelia. Its expression is also a significant marker in several types of cancer, including lung and esophageal cancer, where it can indicate tumor progression and poor prognosis.

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