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验证数据展示

  • WB analysis using 86429-5-RR

    WB analysis using 86429-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86429-5-RR (ALDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of C2C12 using 86429-5-RR

    IF Staining of C2C12 using 86429-5-RR

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using ALDH2 antibody (86429-5-RR, Clone: 251287E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of A549 using 86429-5-RR

    IF Staining of A549 using 86429-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ALDH2 antibody (86429-5-RR, Clone: 251287E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86429-5-RR

    Affinity and Kinetic Characterization of 86429-5-RR

    Biolayer interferometry (BLl) kinetic assays of 86429-5-RR against Human ALDH2 were performed. The affinity constant is below 1 pM.

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ALDH2 Recombinant monoclonal antibody

ALDH2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86429-5-RR

产品说明书

CloneNo. 251287E9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

ALDH 2, ALDH class 2, ALDH E2, ALDHE2, ALDH-E2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, SK-BR-3 cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IF/ICC detected inC2C12 cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy ALDH2 Ready-To-Use IHC Kit. ALDH2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86429-5-RR targets ALDH2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7452

Product name: Recombinant human ALDH2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 165-517 aa of BC002967

Sequence: GDFFSYTRHEPVGVCGQIIPWNFPLLMQAWKLGPALATGNVVVMKVAEQTPLTALYVANLIKEAGFPPGVVNIVPGFGPTAGAAIASHEDVDKVAFTGSTEIGRVIQVAAGSSNLKRVTLELGGKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDIYDEFVERSVARAKSRVVGNPFDSKTEQGPQVDETQFKKILGYINTGKQEGAKLLCGGGIAADRGYFIQPTVFGDVQDGMTIAKEEIFGPVMQILKFKTIEEVVGRANNSTYGLAAAVFTKDLDKANYLSQALQAGTVWVNCYDVFGAQSPFGGYKMSGSGRELGEYGLQAYTEVKTVTVKVPQKNS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aldehyde dehydrogenase 2 family (mitochondrial)
别名 ALDH 2, ALDH class 2, ALDH E2, ALDHE2, ALDH-E2
计算分子量 56 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC002967
基因名称 ALDH2
Gene ID (NCBI) 217
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05091
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.

实验方案

Product Specific Protocols
WB protocol for ALDH2 antibody 86429-5-RRDownload protocol
IF protocol for ALDH2 antibody 86429-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols