×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 86368-1-RR (same clone as 86368-1-PBS)

    WB analysis using 86368-1-RR (same clone as 86368-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86368-1-RR (PRDX6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86368-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02551-1

    Sandwich ELISA sample test of MP02551-1

    Plasma of six individual healthy human donors was measured. The human PRDX6 concentration of detected samples was determined to be 0.80 ng/mL with a range of 0.27 - 1.25 ng/mL.

  • Sandwich ELISA standard curve of MP02551-1

    Sandwich ELISA standard curve of MP02551-1

    Sandwich ELISA standard curve of MP02551-1, Human PRDX6 Recombinant Matched Antibody Pair - PBS only. 86368-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4512. 86368-3-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • IF Staining of K-562 using 86368-1-RR (same clone as 86368-1-PBS)

    IF Staining of K-562 using 86368-1-RR (same clone as 86368-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using PRDX6 antibody (86368-1-RR, Clone: 251053E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86368-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86368-1-RR

    Affinity and Kinetic Characterization of 86368-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86368-1-RR against Human PRDX6 were performed. The affinity constant is 0.234 nM.

PRDX6 Recombinant monoclonal antibody, PBS Only (Capture)

PRDX6 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA
Cat No. 86368-1-PBS

产品说明书

CloneNo. 251053E8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

别名

1-Cys peroxiredoxin, 1-Cys PRX, Acidic calcium-independent phospholipase A2, AOP2, EC:1.11.1.27

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy PRDX6 Ready-To-Use IHC Kit. PRDX6 primary antibody included.

产品信息

86368-1-PBS targets PRDX6 as part of a matched antibody pair:

MP02551-1: 86368-1-PBS capture and 86368-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4512

Product name: Recombinant human PRDX6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-224 aa of BC035857

Sequence: MPGGLLLGDVAPNFEANTTVGRIRFHDFLGDSWGILFSHPRDFTPVCTTELGRAAKLAPEFAKRNVKLIALSIDSVEDHLAWSKDINAYNCEEPTEKLPFPIIDDRNRELAILLGMLDPAEKDEKGMPVTARVVFVFGPDKKLKLSILYPATTGRNFDEILRVVISLQLTAEKRVATPVDWKDGDSVMVLPTIPEEEAKKLFPKGVFTKELPSGKKYLRYTPQP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 peroxiredoxin 6
别名 1-Cys peroxiredoxin, 1-Cys PRX, Acidic calcium-independent phospholipase A2, AOP2, EC:1.11.1.27
计算分子量 224 aa, 25 kDa
观测分子量25-28 kDa
GenBank蛋白编号BC035857
基因名称 PRDX6
Gene ID (NCBI) 9588
RRIDAB_3744844
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP30041
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).

||
New chat

Able™

正在加载，请稍候...