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验证数据展示

  • WB analysis using 86345-2-RR (same clone as 86345-2-PBS)

    WB analysis using 86345-2-RR (same clone as 86345-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86345-2-RR (ATP6V1C1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86345-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 86345-2-RR (same clone as 86345-2-PBS)

    IHC staining of mouse brain using 86345-2-RR (same clone as 86345-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86345-2-RR (ATP6V1C1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86345-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 86345-2-RR (same clone as 86345-2-PBS)

    IHC staining of mouse brain using 86345-2-RR (same clone as 86345-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86345-2-RR (ATP6V1C1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86345-2-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 86345-2-RR (same clone as 86345-2-PBS)

    IF Staining of NIH/3T3 using 86345-2-RR (same clone as 86345-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using ATP6V1C1 antibody (86345-2-RR, Clone: 250958C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86345-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86345-2-RR (same clone as 86345-2-PBS)

    IF Staining of HeLa using 86345-2-RR (same clone as 86345-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATP6V1C1 antibody (86345-2-RR, Clone: 250958C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86345-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02350-1

    Cytometric bead array sample test of MP02350-1

    The mean ATP6V1C1 concentration was determined to be 29.6 ng/mL in NIH/3T3 cell extract based on a 1.4 mg/mL extract load.

  • Cytometric bead array standard curve of MP02350-1

    Cytometric bead array standard curve of MP02350-1

    Cytometric bead array standard curve of MP02350-1, ATP6V1C1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86345-2-PBS. Detection antibody: 86345-1-PBS. Standard: Ag9020. Range: 1.562-200 ng/mL.

  • Affinity and Kinetic Characterization of 86345-2-RR

    Affinity and Kinetic Characterization of 86345-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86345-2-RR against Human ATP6V1C1 were performed. The affinity constant is 8.06 nM.

ATP6V1C1 Recombinant monoclonal antibody, PBS Only (Capture)

ATP6V1C1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86345-2-PBS

产品说明书

CloneNo. 250958C3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

ATP6C, ATP6D, VATC, V-ATPase subunit C 1, Vma5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86345-2-PBS targets ATP6V1C1 as part of a matched antibody pair:

MP02350-1: 86345-2-PBS capture and 86345-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9020

Product name: Recombinant human ATP6V1C1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-381 aa of BC010960

Sequence: MTEFWLISAPGEKTCQQTWEKLHAATSKNNNLAVTSKFNIPDLKVGTLDVLVGLSDELAKLDAFVEGVVKKVAQYMADVLEDSKDKVQENLLANGVDLVTYITRFQWDMAKYPIKQSLKNISEIIAKGVTQIDNDLKSRASAYNNLKGNLQNLERKNAGSLLTRSLAEIVKKDDFVLDSEYLVTLLVVVPKLNHNDWIKQYETLAEMVVPRSSNVLSEDQDSYLCNVTLFRKAVDDFRHKARENKFIVRDFQYNEEEMKADKEEMNRLSTDKKKQFGPLVRWLKVNFSEAFIAWIHVKALRVFVESVLRYGLPVNFQAMLLQPNKKTLKKLREVLHELYKHLDSSAAAIIDAPMDIPGLNLSQQEYYPYVYYKIDCNLLEF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1
别名 ATP6C, ATP6D, VATC, V-ATPase subunit C 1, Vma5
计算分子量 382 aa, 44 kDa
观测分子量 42-44 kDa
GenBank蛋白编号BC010960
基因名称 ATP6V1C1
Gene ID (NCBI) 528
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP21283
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The vacuolar H+-ATPase (V-ATPase) is a functionally conserved multimeric complex localized at the membranes of many organelles where its proton-pumping action is required for proper lumen acidification (PMID: 39210597). ATP6V1C1, also known as ATPase, H+ transporting, lysosomal V1 subunit C1, is a component of the V1 sector of the V-ATPase complex. ATP6v1c1 knockdown significantly reduces tumor stimulated bone resorption through osteoclastogenesis at the bone and metastasis in vivo, as well as V-ATPase activity, proliferation, and mTORC1 activation in vitro (PMID: 28504970). Also, ATP6V1C1, associated with the tumor microenvironment and mTORC1 signaling pathway, is a potential diagnostic, prognostic, and therapeutic biomarker for hepatocellular carcinoma () (PMID: 39557733).

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