• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 86334-2-RR (same clone as 86334-2-PBS)

    WB analysis using 86334-2-RR (same clone as 86334-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86334-2-RR (HSD17B4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86334-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86334-2-RR (same clone as 86334-2-PBS)

    IF Staining of HeLa using 86334-2-RR (same clone as 86334-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSD17B4 antibody (86334-2-RR, Clone: 251058E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86334-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02361-1

    Cytometric bead array sample test of MP02361-1

    The mean HSD17B4 concentration was determined to be 3562.2 ng/mL in Hela cell extract based on a 1.5 mg/mL extract load.

  • Cytometric bead array standard curve of MP02361-1

    Cytometric bead array standard curve of MP02361-1

    Cytometric bead array standard curve of MP02361-1, HSD17B4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86334-2-PBS. Detection antibody: 86334-1-PBS. Standard: Ag7165. Range: 1.562-200 ng/mL.

  • Affinity and Kinetic Characterization of 86334-2-RR

    Affinity and Kinetic Characterization of 86334-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86334-2-RR against Human HSD17B4 were performed. The affinity constant is below 1 pM.

HSD17B4 Recombinant monoclonal antibody, PBS Only (Capture)

HSD17B4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86334-2-PBS

产品说明书

CloneNo. 251058E1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

(3R)-hydroxyacyl-CoA dehydrogenase, 17 beta HSD 4, 17-beta-HSD 4, 17-beta-hydroxysteroid dehydrogenase 4, D bifunctional protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy HSD17B4 Ready-To-Use IHC Kit. HSD17B4 primary antibody included.

产品信息

86334-2-PBS targets HSD17B4 as part of a matched antibody pair:

MP02361-1: 86334-2-PBS capture and 86334-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag7165

Product name: Recombinant human HSD17B4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 436-736 aa of BC003098

Sequence: GSGVVIIMDVYSYSEKELICHNQFSLFLVGSGGFGGKRTSDKVKVAVAIPNRPPDAVLTDTTSLNQAALYRLSGDWNPLHIDPNFASLAGFDKPILHGLCTFGFSARRVLQQFADNDVSRFKAIKARFAKPVYPGQTLQTEMWKEGNRIHFQTKVQETGDIVISNAYVDLAPTSGTSAKTPSEGGKLQSTFVFEEIGRRLKDIGPEVVKKVNAVFEWHITKGGNIGAKWTIDLKSGSGKVYQGPAKGAADTTIILSDEDFMEVVLGKLDPQKAFFSGRLKARGNIMLSQKLQMILKDYAKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hydroxysteroid (17-beta) dehydrogenase 4
别名 (3R)-hydroxyacyl-CoA dehydrogenase, 17 beta HSD 4, 17-beta-HSD 4, 17-beta-hydroxysteroid dehydrogenase 4, D bifunctional protein
计算分子量 80 kDa
观测分子量75 kDa, 45 kDa
GenBank蛋白编号BC003098
基因名称 HSD17B4
Gene ID (NCBI) 3295
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP51659
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSD17B4 (17-beta-hydroxysteroid dehydrogenase 4) is also named as Peroxisomal multifunctional enzyme type 2, D-bifuntional protein or multifunctional protein 2. It codes for a 80 kDa enzyme containing three distinct functional domains and is localized in peroxisomes. It is a bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids and catalyzing the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-brancked-chian fatty acids. After peroxisomal import, the full-length protein is proteolytically cleaved to yield a 35-kDa dehydrogenase subunit and a 45-kDa hydratase subunit containing the hydratase and SCP domains (PMID: 28868548, 24602372).

||
New chat

Able™

正在加载，请稍候...