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验证数据展示

  • WB analysis of mouse skin using 86310-1-RR

    WB analysis of mouse skin using 86310-1-RR

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 86310-1-RR (KRT79 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skin using 86310-1-RR

    IHC staining of mouse skin using 86310-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 86310-1-RR (Cytokeratin 79 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 86310-1-RR

    IHC staining of human skin cancer using 86310-1-RR

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86310-1-RR (Cytokeratin 79 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse skin using 86310-1-RR

    IF Staining of mouse skin using 86310-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 79 antibody (86310-1-RR, Clone: 250927D8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cytokeratin 79 Recombinant monoclonal antibody

Cytokeratin 79 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 86310-1-RR

产品说明书

CloneNo. 250927D8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

CK-79, Cytokeratin-79, K6L, K79, KB38

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skin tissue
Positive IHC detected inhuman skin cancer tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skin tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86310-1-RR targets Cytokeratin 79 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratin 79
别名 CK-79, Cytokeratin-79, K6L, K79, KB38
观测分子量58 kDa
GenBank蛋白编号BC039148
基因名称 KRT79
Gene ID (NCBI) 338785
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ5XKE5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KRT79(Keratin 79, also known as K6L or KRT6L) is a type II epithelial keratin, which is mainly expressed in skeletal muscle, skin, scalp and other tissues and participates in maintaining the structural integrity of epithelial cells. KRT79 plays an important role in hair follicle development, and it is one of the marker genes of hair follicle outer root sheath and hair stromal cells (HG cells). Together with KRT17 and SOX9, it marks HG cells and participates in hair follicle regeneration and morphogenesis.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 79 antibody 86310-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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