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验证数据展示

  • WB analysis using 86305-2-RR (same clone as 86305-2-PBS)

    WB analysis using 86305-2-RR (same clone as 86305-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86305-2-RR (L1CAM/CD171 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86305-2-PBS in a different storage buffer formulation.

  • IF Staining of Neuro-2a using 86305-2-RR (same clone as 86305-2-PBS)

    IF Staining of Neuro-2a using 86305-2-RR (same clone as 86305-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using L1CAM/CD171 antibody (86305-2-RR, Clone: 250101C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86305-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 86305-2-RR (same clone as 86305-2-PBS)

    IHC staining of mouse kidney using 86305-2-RR (same clone as 86305-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86305-2-RR (L1CAM/CD171 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86305-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 86305-2-RR (same clone as 86305-2-PBS)

    IHC staining of mouse kidney using 86305-2-RR (same clone as 86305-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86305-2-RR (L1CAM/CD171 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86305-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02352-1

    Cytometric bead array sample test of MP02352-1

    The mean L1CAM concentration was determined to be 269.5 ng/mL in mouse serum.

  • Cytometric bead array standard curve of MP02352-1

    Cytometric bead array standard curve of MP02352-1

    Cytometric bead array standard curve of MP02352-1, MOUSE L1CAM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86305-2-PBS. Detection antibody: 86305-3-PBS. Standard: Eg3022. Range: 0.781-100 ng/mL.

Mouse L1CAM/CD171 Recombinant monoclonal antibody, PBS Only (Capture)

L1CAM/CD171 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86305-2-PBS

产品说明书

CloneNo. 250101C11

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

L1cam, N-CAM-L1, CAML1, CD171, N CAM L1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86305-2-PBS targets L1CAM/CD171 as part of a matched antibody pair:

MP02352-1: 86305-2-PBS capture and 86305-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg3022

Product name: Recombinant Mouse L1cam protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 20-1123 aa of N/A

Sequence: IQIPDEYKGHHVLEPPVITEQSPRRLVVFPTDDISLKCEARGRPQVEFRWTKDGIHFKPKEELGVVVHEAPYSGSFTIEGNNSFAQRFQGIYRCYASNKLGTAMSHEIQLVAEGAPKWPKETVKPVEVEEGESVVLPCNPPPSAAPPRIYWMNSKIFDIKQDERVSMGQNGDLYFANVLTSDNHSDYICNAHFPGTRTIIQKEPIDLRVKPTNSMIDRKPRLLFPTNSSSRLVALQGQSLILECIAEGFPTPTIKWLHPSDPMPTDRVIYQNHNKTLQLLNVGEEDDGEYTCLAENSLGSARHAYYVTVEAAPYWLQKPQSHLYGPGETARLDCQVQGRPQPEITWRINGMSMETVNKDQKYRIEQGSLILSNVQPTDTMVTQCEARNQHGLLLANAYIYVVQLPARILTKDNQTYMAVEGSTAYLLCKAFGAPVPSVQWLDEEGTTVLQDERFFPYANGTLSIRDLQANDTGRYFCQAANDQNNVTILANLQVKEATQITQGPRSAIEKKGARVTFTCQASFDPSLQASITWRGDGRDLQERGDSDKYFIEDGKLVIQSLDYSDQGNYSCVASTELDEVESRAQLLVVGSPGPVPHLELSDRHLLKQSQVHLSWSPAEDHNSPIEKYDIEFEDKEMAPEKWFSLGKVPGNQTSTTLKLSPYVHYTFRVTAINKYGPGEPSPVSESVVTPEAAPEKNPVDVRGEGNETNNMVITWKPLRWMDWNAPQIQYRVQWRPQGKQETWRKQTVSDPFLVVSNTSTFVPYEIKVQAVNNQGKGPEPQVTIGYSGEDYPQVSPELEDITIFNSSTVLVRWRPVDLAQVKGHLKGYNVTYWWKGSQRKHSKRHIHKSHIVVPANTTSAILSGLRPYSSYHVEVQAFNGRGLGPASEWTFSTPEGVPGHPEALHLECQSDTSLLLHWQPPLSHNGVLTGYLLSYHPVEGESKEQLFFNLSDPELRTHNLTNLNPDLQYRFQLQATTQQGGPGEAIVREGGTMALFGKPDFGNISATAGENYSVVSWVPRKGQCNFRFHILFKALPEGKVSPDHQPQPQYVSYNQSSYTQWNLQPDTKYEIHLIKEKVLLHHLDVKTNGTGPVRVSTTGSFASE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 L1 cell adhesion molecule
别名 L1cam, N-CAM-L1, CAML1, CD171, N CAM L1
计算分子量141 kDa
观测分子量210-250 kDa
GenBank蛋白编号N/A
基因名称 L1cam
Gene ID (NCBI) 16728
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP11627
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310).

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