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验证数据展示

  • WB analysis using 86272-1-RR (same clone as 86272-1-PBS)

    WB analysis using 86272-1-RR (same clone as 86272-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86272-1-RR (FNDC3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86272-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 86272-1-RR (same clone as 86272-1-PBS)

    IHC staining of human breast cancer using 86272-1-RR (same clone as 86272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86272-1-RR (FNDC3B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86272-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 86272-1-RR (same clone as 86272-1-PBS)

    IF Staining of HepG2 using 86272-1-RR (same clone as 86272-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FNDC3B antibody (86272-1-RR, Clone: 250827H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86272-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02305-1

    Cytometric bead array sample test of MP02305-1

    The mean FNDC3B concentration was determined to be 70.0 ng/mL in HepG2 cell extract based on a 0.9 mg/mL extract load.

  • Cytometric bead array standard curve of MP02305-1

    Cytometric bead array standard curve of MP02305-1

    Cytometric bead array standard curve of MP02305-1, FNDC3B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86272-2-PBS. Detection antibody: 86272-1-PBS. Standard: Ag18357. Range: 3.125-200 ng/mL.

FNDC3B Recombinant monoclonal antibody, PBS Only (Detector)

FNDC3B Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86272-1-PBS

产品说明书

CloneNo. 250827H1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

FAD104, fibronectin type III domain containing 3B, Fibronectin type III domain-containing protein 3B, HCV NS5A-binding protein 37, UNQ2421/PRO4979/PRO34274

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86272-1-PBS targets FNDC3B as part of a matched antibody pair:

MP02305-1: 86272-2-PBS capture and 86272-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18357

Product name: Recombinant human FNDC3B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 3-370 aa of BC039297

Sequence: VTMMMTDQIPLELPPLLNGEVAMMPHLVNGDAAQQVILVQVNPGETFTIRAEDGTLQCIQGPAEVPMMSPNGSIPPIHVPPGYISQVIEDSTGVRRVVVTPQSPECYPPSYPSAMSPTHHLPPYLTHHPHFIHNSHTAYYPPVTGPGDMPPQFFPQHHLPHTIYGEQEIIPFYGMSSYITREDQYSKPPHKKLKDRQIDRQNRLNSPPSSIYKSSCTTVYNGYGKGHSGGSGGGGSGSGPGIKKTERRARSSPKSNDSDLQEYELEVKRVQDILSGIEKPQVSNIQARAVVLSWAPPVGLSCGPHSGLSFPYSYEVALSDKGRDGKYKIIYSGEELECNLKDLRPATDYHVRVYAMYNSVKGSCSEPV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fibronectin type III domain containing 3B
别名 FAD104, fibronectin type III domain containing 3B, Fibronectin type III domain-containing protein 3B, HCV NS5A-binding protein 37, UNQ2421/PRO4979/PRO34274
计算分子量 1204 aa, 133 kDa
观测分子量 150 kDa
GenBank蛋白编号BC039297
基因名称 FNDC3B
Gene ID (NCBI) 64778
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ53EP0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fibronectin type III domain-containing protein 3B(FNDC3B), also named FAD104, belongs to the FNDC3 family. NDC3B is a positive regulator of adipocyte differentiation and is expressed at an early stage of adipocyte differentiation. FNDC3B is an endoplasmic reticulum transmembrane protein with a single transmembrane domain at the C terminus preceded by nine repeated fibronectin type III domains(PMID: 32966780). Due to the ability of the fibronectin type III domain to bind to a variety of proteins, FNDC3B plays a crucial role in cell adhesion, proliferation, and growth signaling. FNDC3B is abnormally expressed in a variety of human cancers, including hepatocellular carcinoma, acute myeloid leukemia, colorectal carcinoma, and cervical carcinoma(PMID: 36275754).

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