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验证数据展示

  • WB analysis using 86257-3-RR

    WB analysis using 86257-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86257-3-RR (JAKMIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 86257-3-RR

    IF Staining of Jurkat using 86257-3-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using JAKMIP1 antibody (86257-3-RR, Clone: 251026C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HEK-293 using 86257-3-RR

    IF Staining of HEK-293 using 86257-3-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using JAKMIP1 antibody (86257-3-RR, Clone: 251026C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86257-3-RR

    Affinity and Kinetic Characterization of 86257-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86257-3-RR against Human JAKMIP1 were performed. The affinity constant is below 1 pM.

JAKMIP1 Recombinant monoclonal antibody

JAKMIP1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86257-3-RR

产品说明书

CloneNo. 251026C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

GABA-B receptor-binding protein, GABABRBP, JAMIP1, janus kinase and microtubule interacting protein 1, Janus kinase and microtubule-interacting protein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IF/ICC detected inHEK-293 cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86257-3-RR targets JAKMIP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4816

Product name: Recombinant human JAKMIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 277-626 aa of BC047075

Sequence: VQDQHMDERDVRRFQLKIAELNSVIRKLEDRNTLLADERNELLKRSRETEVQLKPLVEKNKRMNKKNEDLLQSIQRMEEKIKNLTRENVEMKEKLSAQASLKRHTSLNDLSLTRDEQEIEFLRLQVLEQQHVIDDLSLERERLLRSKRHRGKSLKPPKKHVVETFFGFDEESVDSETLSETSYNTDRTDRTPATPEEDLDDATAREEADLRFCQLTREYQALQRAYALLQEQVGGTLDAEREARTREQLQADLLRCQAKIEDLEKLLVEKGQDSKWVEEKQLLIRTNQDLLEKIYRLEMEENQLKNEMQDAKDQNELLEFRVLELEVRDSICCKLSNGADILFEPKLKFM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 janus kinase and microtubule interacting protein 1
别名 GABA-B receptor-binding protein, GABABRBP, JAMIP1, janus kinase and microtubule interacting protein 1, Janus kinase and microtubule-interacting protein 1
计算分子量 73 kDa
观测分子量 73-75 kDa
GenBank蛋白编号BC047075
基因名称 JAKMIP1
Gene ID (NCBI) 152789
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96N16
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for JAKMIP1 antibody 86257-3-RRDownload protocol
WB protocol for JAKMIP1 antibody 86257-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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