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验证数据展示

  • WB analysis using 86218-2-RR

    WB analysis using 86218-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86218-2-RR (ATP5I antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 86218-2-RR

    IF Staining of HeLa using 86218-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5I antibody (86218-2-RR, Clone: 250815C6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

ATP5I Recombinant antibody

ATP5I Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86218-2-RR

产品说明书

CloneNo. 250815C6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

ATP synthase membrane subunit e, ATP synthase subunit e, mitochondrial, N-terminally processed, ATP5K, ATP5ME, ATPase subunit e

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, U-251 cells, A431 cells, human heart tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86218-2-RR targets ATP5I in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ATP5I fusion protein Ag9605 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E
别名 ATP synthase membrane subunit e, ATP synthase subunit e, mitochondrial, N-terminally processed, ATP5K, ATP5ME, ATPase subunit e
计算分子量 69 aa, 8 kDa
观测分子量 8 kDa
GenBank蛋白编号BC003679
基因名称 ATP5I
Gene ID (NCBI) 521
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP56385
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP5I(ATP synthase subunit e) is also named as ATP5K and belongs to the ATPase e subunit family. The ATP5I gene encodes the e subunit of the mitochondrial ATP synthase Fo complex. Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. Antisense ATP5I in a human HCC cell line inhibited cell growth suggesting that ATP5I acts through the MAP kinase pathway(PMID:11939412).

实验方案

Product Specific Protocols
WB protocol for ATP5I antibody 86218-2-RRDownload protocol
IF protocol for ATP5I antibody 86218-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols