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验证数据展示

  • WB analysis of HepG2 using 86178-1-RR

    WB analysis of HepG2 using 86178-1-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 86178-1-RR (CA13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86178-1-RR

    WB analysis using 86178-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86178-1-RR (CA13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 86178-1-RR

    WB analysis of NIH/3T3 using 86178-1-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86178-1-RR (CA13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CA13 Recombinant antibody

CA13 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 86178-1-RR

产品说明书

CloneNo. 250787A12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

CA XIII, Carbonate dehydratase XIII, Carbonic anhydrase 13, Carbonic anhydrase XIII, CAXIII

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, mouse thymus tissue, NIH/3T3 cells, rat small intestine tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86178-1-RR targets CA13 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CA13 fusion protein Ag10086 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 carbonic anhydrase XIII
别名 CA XIII, Carbonate dehydratase XIII, Carbonic anhydrase 13, Carbonic anhydrase XIII, CAXIII
计算分子量 262 aa, 29 kDa
观测分子量30 kDa
GenBank蛋白编号BC052602
基因名称 CA13
Gene ID (NCBI) 377677
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N1Q1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carbonic anhydrases (CAs) are zinc-containing metalloenzymes that catalyze the reversible hydration of carbon dioxide. The mammalian α-CA gene family has been reported to include at least eleven enzymatically active isoforms with different structural and catalytic properties. Four of the active CA isozymes are cytosolic (CA I, II, III, and VII), four are membrane-associated(CA IV, IX, XII, and XIV), two are mitochondrial (CA VA and VB), and one is a secretory form (CA VI). CA13 is a cytosolic protein that may play a role in embryogenesis and perturbation of its function by genetic modification could potentially lead to developmental abnormalities(PMID:14600151).

实验方案

Product Specific Protocols
WB protocol for CA13 antibody 86178-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols