验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, mouse testis tissue, U-251 cells, BxPC-3 cells, HaCaT cells, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86164-2-RR targets REEP4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | REEP4 fusion protein Ag24569 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | receptor accessory protein 4 |
| 别名 | C8orf20, PP432, Receptor expression-enhancing protein 4 |
| 计算分子量 | 29 kDa |
| 观测分子量 | 29 kDa |
| GenBank蛋白编号 | BC013048 |
| 基因名称 | REEP4 |
| Gene ID (NCBI) | 80346 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9H6H4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
REEP4 (Receptor Expression-Enhancing Protein 4) is a member of the REEP family of proteins, a transmembrane protein that interacts with odorant receptor proteins and may enhance the odorant receptor responses to odorants. It is localized to the endoplasmic reticulum (ER) and is associated with the nuclear envelope (NE). REEP4 helps link the high-curvature ER to the ELYS-based nuclear pore complex (NPC) seed, thereby promoting NPC biogenesis during late mitosis. REEP4 expression is associated with the cell cycle, osteoclast differentiation, Fc gamma R-mediated phagocytosis, and Human T-cell leukemia virus 1 infection. (PMID: 36191880; 38947393)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for REEP4 antibody 86164-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
