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验证数据展示

  • WB analysis using 86135-1-RR

    WB analysis using 86135-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86135-1-RR (RRM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 86135-1-RR

    IF Staining of HeLa using 86135-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RRM1 antibody (86135-1-RR, Clone: 250707E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RRM1 Recombinant antibody

RRM1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86135-1-RR

产品说明书

CloneNo. 250707E6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

EC:1.17.4.1, Ribonucleoside-diphosphate reductase large subunit, Ribonucleoside-diphosphate reductase subunit M1, ribonucleotide reductase M1, RIR1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, A431 cells, HEK-293 cells, K-562 cells, A549 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RRM1 Ready-To-Use IHC Kit. RRM1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86135-1-RR targets RRM1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 RRM1 fusion protein Ag0789 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribonucleotide reductase M1
别名 EC:1.17.4.1, Ribonucleoside-diphosphate reductase large subunit, Ribonucleoside-diphosphate reductase subunit M1, ribonucleotide reductase M1, RIR1
计算分子量 90 kDa
观测分子量90 kDa
GenBank蛋白编号BC006498
基因名称 RRM1
Gene ID (NCBI) 6240
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP23921
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribonucleoside-diphosphate reductase functions as a heterodimer of a large and a small subunits in deoxyribonucleotide synthesis. RRM1 constitutes to the large subunit (R1) of ribonucleotide reductase, and it can either form heterodimer with small subunit RRM or RRM2B(PMID:16376858). RRM1 provides the precursors necessary for DNA synthesis. RRM1 can not be detected in quiescent cells, while its mRNA and protein are present throughout the cell cycle in cycling cells(PMID:8188248). Researches showed that RRM1 is involved in carcinogenesis, tumor progression, and the resistance of non-small-cell lung cancer (NSCLC) to treatment. Low level expression of RRM1 in NSCLC is associated with poor survival (PMID:17314339).

实验方案

Product Specific Protocols
WB protocol for RRM1 antibody 86135-1-RRDownload protocol
IF protocol for RRM1 antibody 86135-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols