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验证数据展示

  • WB analysis using 86099-1-RR

    WB analysis using 86099-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86099-1-RR (SNRPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 86099-1-RR

    IF Staining of A549 using 86099-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SNRPB antibody (86099-1-RR, Clone: 250577G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SNRPB Recombinant monoclonal antibody

SNRPB Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86099-1-RR

产品说明书

CloneNo. 250577G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Sm protein B/B, Sm protein B/B', Small nuclear ribonucleoprotein associated proteins B and B', Small nuclear ribonucleoprotein-associated proteins B and B', SmB/B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, A549 cells, Jurkat cells, Ramos cells, K-562 cells, NIH/3T3 cells
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy SNRPB Ready-To-Use IHC Kit. SNRPB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86099-1-RR targets SNRPB in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag10298

Product name: Recombinant human SNRPB protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-234 aa of BC003530

Sequence: MTVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDCDEFRKIKPKNSKQAEREEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGIGRAAGRGIPAGVPMPQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAAAATASIAGAPTQYPPGRGGPPPPMGRGAPPPGMMGPPPGMRPPMGPPMGIPPGRGTPMGMPPPGMRPPPPGMRGKCLQW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 small nuclear ribonucleoprotein polypeptides B and B1
别名 Sm protein B/B, Sm protein B/B', Small nuclear ribonucleoprotein associated proteins B and B', Small nuclear ribonucleoprotein-associated proteins B and B', SmB/B
计算分子量234aa,25 kDa; 285aa,30 kDa
观测分子量25-30 kDa
GenBank蛋白编号BC003530
基因名称 SNRPB
Gene ID (NCBI) 6628
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP14678
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNRPB (Small Nuclear Ribonucleoprotein Polypeptides B and B1) is a core component of the spliceosome, a complex molecular machine that plays a critical role in pre-mRNA splicing. This process is essential for the removal of introns and the joining of exons to produce mature mRNA molecules.

实验方案

Product Specific Protocols
IF protocol for SNRPB antibody 86099-1-RRDownload protocol
WB protocol for SNRPB antibody 86099-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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