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验证数据展示

  • WB analysis using 86053-1-RR (same clone as 86053-1-PBS)

    WB analysis using 86053-1-RR (same clone as 86053-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86053-1-RR (CD3 epsilon/CD3e antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

  • IHC staining of rat spleen using 86053-1-RR (same clone as 86053-1-PBS)

    IHC staining of rat spleen using 86053-1-RR (same clone as 86053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 86053-1-RR (CD3 epsilon antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

  • IHC staining of rat spleen using 86053-1-RR (same clone as 86053-1-PBS)

    IHC staining of rat spleen using 86053-1-RR (same clone as 86053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 86053-1-RR (CD3 epsilon antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02251-2

    Sandwich ELISA sample test of MP02251-2

    The mean CD3 epsilon concentration was determined to be 6.3 ng/mL in rat heart tissue extract based on a 6.7 mg/mL extract load and 13.6 ng/mL in rat spleen tissue extract based on a 2.7 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02251-2

    Sandwich ELISA standard curve of MP02251-2

    Sandwich ELISA standard curve of MP02251-2, Rat CD3 epsilon Recombinant Matched Antibody Pair - PBS only. 86053-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1450. 86053-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • IF Staining of rat thymus using 86053-1-RR (same clone as 86053-1-PBS)

    IF Staining of rat thymus using 86053-1-RR (same clone as 86053-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat thymus tissue using CD3 epsilon antibody (86053-1-RR, Clone: 242849D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

  • IF Staining of rat thymus using 86053-1-RR (same clone as 86053-1-PBS)

    IF Staining of rat thymus using 86053-1-RR (same clone as 86053-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat thymus tissue using CD3 epsilon antibody (86053-1-RR, Clone: 242849D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

  • IF Staining of rat spleen using 86053-1-RR (same clone as 86053-1-PBS)

    IF Staining of rat spleen using 86053-1-RR (same clone as 86053-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD3 epsilon antibody (86053-1-RR, Clone: 242849D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

Rat CD3 epsilon Recombinant monoclonal antibody, PBS Only (Detector)

CD3 epsilon Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA
Cat No. 86053-1-PBS

产品说明书

CloneNo. 242849D3

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA

别名

CD3e, T-cell surface glycoprotein CD3 epsilon chain

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86053-1-PBS targets CD3 epsilon as part of a matched antibody pair:

MP02251-2: 86053-3-PBS capture and 86053-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg1450

Product name: Recombinant Rat CD3 epsilon protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 24-103 aa of NP_001101610.2

Sequence: IEDTVTDDGAQKQYEVSISGTSVELTCPLENEDNLKWEKNDKVLPDKNEKHLVLEDFSEVKDSGYYVCYTESSRKNTYLY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD3 molecule, epsilon polypeptide
别名 CD3e, T-cell surface glycoprotein CD3 epsilon chain
计算分子量22kDa
观测分子量22 kDa
GenBank蛋白编号NP_001101610.2
基因名称 Cd3e
Gene ID (NCBI) 315609
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDA6J423
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657). This antibody was raised against the epsilon chain of rat CD3 molecule.

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