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验证数据展示

  • WB analysis using 86050-1-RR

    WB analysis using 86050-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86050-1-RR (SERCA2,ATP2A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 86050-1-RR

    IHC staining of mouse skeletal muscle using 86050-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 86050-1-RR (SERCA2/ATP2A2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP2A2 antibody included
  • IF Staining of C2C12 using 86050-1-RR

    IF Staining of C2C12 using 86050-1-RR

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using SERCA2/ATP2A2 antibody (86050-1-RR, Clone: 250667D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86050-1-RR

    Affinity and Kinetic Characterization of 86050-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86050-1-RR against Human SERCA2/ATP2A2 were performed. The affinity constant is below 1 pM.

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SERCA2/ATP2A2 Recombinant monoclonal antibody

SERCA2/ATP2A2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 86050-1-RR

产品说明书

CloneNo. 250667D7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ATP2A2, SERCA2, SERCA2b, ATP2A 2, ATP2B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected in37°C incubated mouse heart tissue, 37℃ incubated mouse heart, rat heart, 37℃ incubated rat heart
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy ATP2A2 Ready-To-Use IHC Kit. ATP2A2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86050-1-RR targets SERCA2/ATP2A2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26177

Product name: Recombinant human SERCA2,ATP2A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 314-580 aa of BC035588

Sequence: VITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFILDRVEGDTCSLNEFTITGSTYAPIGEVHKDDKPVNCHQYDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTELKGLSKIERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHIRVGSTKVPMTSGVKQKIMSVIREWGSGSDTLRCLALATHDNPLRREEMHLEDS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
别名 ATP2A2, SERCA2, SERCA2b, ATP2A 2, ATP2B
计算分子量 1042 aa, 115 kDa
观测分子量110-115 kDa
GenBank蛋白编号BC035588
基因名称 SERCA2
Gene ID (NCBI) 488
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP16615
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.

实验方案

Product Specific Protocols
WB protocol for SERCA2/ATP2A2 antibody 86050-1-RRDownload protocol
IHC protocol for SERCA2/ATP2A2 antibody 86050-1-RRDownload protocol
IF protocol for SERCA2/ATP2A2 antibody 86050-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols