• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 86009-1-RR

    WB analysis using 86009-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86009-1-RR (RBM47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 86009-1-RR

    IHC staining of human colon using 86009-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86009-1-RR (RBM47 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 86009-1-RR

    IHC staining of human colon using 86009-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86009-1-RR (RBM47 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 86009-1-RR

    IF Staining of A549 using 86009-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using RBM47 antibody (86009-1-RR, Clone: 250436H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86009-1-RR

    Affinity and Kinetic Characterization of 86009-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86009-1-RR against Human RBM47 were performed. The affinity constant is 1.02 nM.

    {{ptg:ProductFeaturesInfo}}

RBM47 Recombinant monoclonal antibody

RBM47 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 86009-1-RR

产品说明书

CloneNo. 250436H8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

NET18, RNA binding motif protein 47, RNA binding protein 47, RNA-binding motif protein 47, RNA-binding protein 47

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inMCF-7 cells, A549 cells, Caco-2 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86009-1-RR targets RBM47 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag20984

Product name: Recombinant human RBM47 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 444-593 aa of BC126261

Sequence: VAIPAIGAQYSMFPAAPAPKMIEDGKIHTVEHMISPIAVQPDPASAAAAAAAAAAAAAAVIPTVSTPPPFQGRPITPVYTVAPNVQRIPTAGIYGASYVPFAAPATATIATLQKNAAAAAAVYGGYAGYIPQAFPAAAIQVPIPDVYQTY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RNA binding motif protein 47
别名 NET18, RNA binding motif protein 47, RNA binding protein 47, RNA-binding motif protein 47, RNA-binding protein 47
计算分子量 593 aa, 64 kDa
观测分子量 64 kDa
GenBank蛋白编号BC126261
基因名称 RBM47
Gene ID (NCBI) 54502
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDA0AV96
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM47, also known as RNA-binding motif protein 47 or RNA-binding protein 47, is a vertebrate-conserved RBP containing three RNA-recognition motifs (RRMs) that perform multifaceted roles in RNA editing, early embryonic development, and cancer.Recent studies suggest that RBM47 functions as a tumor suppressor. It has been shown to suppress the progression of breast and colon cancers. RBM47 represents a molecular switch of cell fate decisions that functions as a regulator of the p53/p21-signaling axis. (PMID: 34499322, PMID: 31511650)

实验方案

Product Specific Protocols
WB protocol for RBM47 antibody 86009-1-RRDownload protocol
IHC protocol for RBM47 antibody 86009-1-RRDownload protocol
IF protocol for RBM47 antibody 86009-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols