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验证数据展示

  • WB analysis using 85934-1-RR

    WB analysis using 85934-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85934-1-RR (SP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85934-1-RR

    Affinity and Kinetic Characterization of 85934-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85934-1-RR against Human SP7 were performed. The affinity constant is 1.10 nM.

SP7 Recombinant antibody

SP7 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85934-1-RR

产品说明书

CloneNo. 250286C5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

osterix, OSX, Sp7 transcription factor, Transcription factor Sp7, Zinc finger protein osterix

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSaos-2 cells, U2OS cells, MG-63 cells, Sp2/0 cells, mouse spinal cord tissue, mouse testis tissue, rat brain tiisue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85934-1-RR targets SP7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SP7 fusion protein Ag29889 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Sp7 transcription factor
别名 osterix, OSX, Sp7 transcription factor, Transcription factor Sp7, Zinc finger protein osterix
计算分子量 45 kDa
观测分子量 45 kDa
GenBank蛋白编号NM_152860
基因名称 SP7
Gene ID (NCBI) 121340
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8TDD2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sp7 is identified in several tissues, including bone, tooth, brain, and the reproductive tract (PMID: 11792318, 33135214, 36881265). In bone, Sp7 is expressed at multiple stages throughout the mesenchymal bone lineage and plays different roles in different osteocytes (PMID: 36881265). SP7 regulates osteoblast differentiation and bone formation through osteoblast target genes by recognizing AT-rich region. In osteocytes, SP7 regulates osteocyte maturation and intracortical remodeling through osteocyte target genes by recognizing TGA(G/T) TCA motif. SP7 also regulates chondrocyte differentiation and endochondral ossification (36881265).

实验方案

Product Specific Protocols
WB protocol for SP7 antibody 85934-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols