验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85885-4-RR targets TAF1B in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | TAF1B fusion protein Ag38211 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa |
| 别名 | RAF1B, RAFI63, RNA polymerase I-specific TBP-associated factor 63 kDa, SL1, TAFI63 |
| 计算分子量 | 588 aa, 69 kDa |
| 观测分子量 | 69 kDa |
| GenBank蛋白编号 | BC018137 |
| 基因名称 | TAF1B |
| Gene ID (NCBI) | 9014 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q53T94 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TAF1B is a component of the RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during transcription initiation such as pre-initiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. It binds to rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex, and possibly directly through its interaction with RRN3 (PMID: 11250903; 11283244; 15970593; 21921199).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TAF1B antibody 85885-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |