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验证数据展示

  • WB analysis using 85857-4-RR (same clone as 85857-4-PBS)

    WB analysis using 85857-4-RR (same clone as 85857-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85857-4-RR (ACTN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85857-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 85857-4-RR (same clone as 85857-4-PBS)

    IHC staining of mouse skeletal muscle using 85857-4-RR (same clone as 85857-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 85857-4-RR (ACTN2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85857-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 85857-4-RR (same clone as 85857-4-PBS)

    IHC staining of mouse heart using 85857-4-RR (same clone as 85857-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 85857-4-RR (ACTN2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85857-4-PBS in a different storage buffer formulation.

  • IF Staining of H9C2 using 85857-4-RR (same clone as 85857-4-PBS)

    IF Staining of H9C2 using 85857-4-RR (same clone as 85857-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using ACTN2 antibody (85857-4-RR, Clone: 250229F6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85857-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02153-1

    Cytometric bead array standard curve of MP02153-1

    Cytometric bead array standard curve of MP02153-1, ACTN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85857-4-PBS. Detection antibody: 85857-3-PBS. Standard: Ag5459. Range: 0.781-100 ng/mL.

ACTN2 Recombinant monoclonal antibody, PBS Only (Capture)

ACTN2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 85857-4-PBS

产品说明书

CloneNo. 250229F6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Actinin alpha 2, actinin, alpha 2, ACTN 2, Alpha actinin 2, Alpha-actinin skeletal muscle isoform 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85857-4-PBS targets ACTN2 as part of a matched antibody pair:

MP02153-1: 85857-4-PBS capture and 85857-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5459

Product name: Recombinant human ACTN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of BC051770

Sequence: MNQIEPGVQYNYVYDEDEYMIQEEEWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPKPDRGKMRFHKIANVNKALDYIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNIQNFHTSWKDGLGLCALIHRHRPDLIDYSKLNKDDPIGNINLAMEIAEKHLDIPKMLDAEDIVNTPKPDERAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENERLMEEYERLASELLEWIRRTI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 actinin, alpha 2
别名 Actinin alpha 2, actinin, alpha 2, ACTN 2, Alpha actinin 2, Alpha-actinin skeletal muscle isoform 2
计算分子量 104 kDa
观测分子量103 kDa
GenBank蛋白编号BC051770
基因名称 ACTN2
Gene ID (NCBI) 88
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35609
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha actinin 2 (ACTN2) belongs to the alpha-actinin family and is expressed in both skeletal and cardiac muscles, and functions to anchor myofibrillar actin thin filaments and titin to Z-discs (PMID: 30701273). ACTN2 is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Mutations in ACTN2 are associated with hypertrophic cardiomyopathy, as well as dilated cardiomyopathy and endocardial fibroelastosis (PMID: 20022194, 14567970).

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