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验证数据展示

  • WB analysis using 85848-1-RR (same clone as 85848-1-PBS)

    WB analysis using 85848-1-RR (same clone as 85848-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85848-1-RR (ASF1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85848-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85848-1-RR (same clone as 85848-1-PBS)

    IF Staining of HeLa using 85848-1-RR (same clone as 85848-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASF1B antibody (85848-1-RR, Clone: 250195A7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85848-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 85848-1-RR (same clone as 85848-1-PBS)

    IF Staining of A431 using 85848-1-RR (same clone as 85848-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ASF1B antibody (85848-1-RR, Clone: 250195A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85848-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 85848-1-RR (same clone as 85848-1-PBS)

    IF Staining of HepG2 using 85848-1-RR (same clone as 85848-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ASF1B antibody (85848-1-RR, Clone: 250195A7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85848-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02155-2

    Cytometric bead array standard curve of MP02155-2

    Cytometric bead array standard curve of MP02155-2, ASF1B-specific Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85848-3-PBS. Detection antibody: 85848-1-PBS. Standard: Ag17763. Range: 0.781-50 ng/mL.

  • Affinity and Kinetic Characterization of 85848-1-RR

    Affinity and Kinetic Characterization of 85848-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85848-1-RR against Human ASF1B were performed. The affinity constant is 57.7 nM.

ASF1B Recombinant monoclonal antibody, PBS Only (Detector)

ASF1B Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 85848-1-PBS

产品说明书

CloneNo. 250195A7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

ASF1A/B, Anti-silencing function protein 1 homolog B, CCG1-interacting factor A-II, CIA II, CIA-II

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ASF1B Ready-To-Use IHC Kit. ASF1B primary antibody included.

产品信息

85848-1-PBS targets ASF1B as part of a matched antibody pair:

MP02155-2: 85848-3-PBS capture and 85848-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag17763

Product name: Recombinant human ASF1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 151-202 aa of BC010014

Sequence: INWDNNMDRLEAIETQDPSLGCGLPLNCTPIKGLGLPGCIPGLLPENSMDCI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ASF1 anti-silencing function 1 homolog B (S. cerevisiae)
别名 ASF1A/B, Anti-silencing function protein 1 homolog B, CCG1-interacting factor A-II, CIA II, CIA-II
计算分子量 202 aa, 22 kDa
观测分子量19 kDa
GenBank蛋白编号BC010014
基因名称 ASF1B
Gene ID (NCBI) 55723
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NVP2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly.This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. This antibody reacts with the ASF1B and ASF1A protein.

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