验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue, mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85811-2-RR targets DOPA decarboxylase/DDC in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | DOPA decarboxylase/DDC fusion protein Ag0219 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | dopa decarboxylase (aromatic L-amino acid decarboxylase) |
| 别名 | DDC, DOPA decarboxylase, AADC, Aromatic-L-amino-acid decarboxylase, EC:4.1.1.28 |
| 计算分子量 | 54 kDa |
| 观测分子量 | 48-50 kDa |
| GenBank蛋白编号 | BC008366 |
| 基因名称 | DOPA decarboxylase |
| Gene ID (NCBI) | 1644 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P20711 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DOPA decarboxylase (DDC), also known as aromatic l-amino acid decarboxylase, belongs to the pyridoxal-dependent aminotransferase superfamily. DDC is an enzyme that converts levodopa into dopamine14, the latter being severely depleted in LBD due to the loss of dopaminergic neurons in the substantia nigra (PMID: 3374198, PMID: 28100251). DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. DDC is the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). Researches showed that Ddc is only one of the enzymes in the biosynthetic pathways for bioamines and catecholamines.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DOPA decarboxylase/DDC antibody 85811-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |