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验证数据展示

  • WB analysis using 85800-7-RR

    WB analysis using 85800-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85800-7-RR (hCG Beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 85800-7-RR

    IHC staining of human placenta using 85800-7-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85800-7-RR (hCG Beta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 85800-7-RR

    IHC staining of human placenta using 85800-7-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85800-7-RR (hCG Beta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

hCG Beta Recombinant monoclonal antibody

hCG Beta Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85800-7-RR

产品说明书

CloneNo. 250118E6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CGB, CGB,hCG, CG beta, CGB3, CGbeta

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJAR cells, human placenta tisse
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85800-7-RR targets hCG Beta in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg3378

Product name: Recombinant Human hCG Beta protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 21-165 aa of

Sequence: SKEPLRPRCRPINATLAVEKEGCPVCITVNTTICAGYCPTMTRVLQGVLPALPQVVCNYRDVRFESIRLPGCPRGVNPVVSYAVALSCQCALCRRSTTDCGGPKDHPLTCDDPRFQDSSSSKAPPPSLPSPSRLPGPSDTPILPQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hCG Beta
别名 CGB, CGB,hCG, CG beta, CGB3, CGbeta
计算分子量18 kDa
观测分子量20-25 kDa
基因名称
Gene ID (NCBI) 1082
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP0DN86
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta-hCG (beta-human chorionic gonadotropin) is a glycoprotein hormone produced by the placenta during pregnancy. It is a critical marker used in both obstetrics and oncology for various diagnostic and monitoring purposes. hCG is composed of two subunits: an alpha subunit (similar to other glycoprotein hormones like LH, FSH, and TSH) and a unique beta subunit (β-hCG). The beta subunit is the primary focus for diagnostic tests due to its specificity. β-hCG is initially secreted by the syncytiotrophoblast cells surrounding the developing embryo and later by the placenta.

实验方案

Product Specific Protocols
IHC protocol for hCG Beta antibody 85800-7-RRDownload protocol
WB protocol for hCG Beta antibody 85800-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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