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验证数据展示

  • WB analysis using 85796-5-RR

    WB analysis using 85796-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85796-5-RR (SPR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 85796-5-RR

    IF Staining of HepG2 using 85796-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SPR antibody (85796-5-RR, Clone: 243138A12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85796-5-RR

    Affinity and Kinetic Characterization of 85796-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85796-5-RR against Human SPR were performed. The affinity constant is 0.855 nM.

SPR Recombinant antibody

SPR Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85796-5-RR

产品说明书

CloneNo. 243138A12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

EC:1.1.1.153, SDR38C1, Sepiapterin reductase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HepG2 cells, HeLa cells, MCF-7 cells, A-549 cells, human skeletal muscle tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85796-5-RR targets SPR in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SPR fusion protein Ag10395 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase)
别名 EC:1.1.1.153, SDR38C1, Sepiapterin reductase
计算分子量 261 aa, 28 kDa
观测分子量28 kDa
GenBank蛋白编号BC017310
基因名称 SPR
Gene ID (NCBI) 6697
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35270
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SPR antibody 85796-5-RRDownload protocol
IF protocol for SPR antibody 85796-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols