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验证数据展示

  • WB analysis using 85791-4-RR

    WB analysis using 85791-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85791-4-RR (FAM134A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 85791-4-RR

    IHC staining of mouse testis using 85791-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85791-4-RR (FAM134A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 85791-4-RR

    IHC staining of mouse testis using 85791-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85791-4-RR (FAM134A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85791-4-RR

    Affinity and Kinetic Characterization of 85791-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85791-4-RR against Human FAM134A were performed. The affinity constant is 3.17 nM.

FAM134A Recombinant antibody

FAM134A Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85791-4-RR

产品说明书

CloneNo. 250097C7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

C2orf17, MAG2, Protein FAM134A, Reticulophagy regulator 2, RETREG2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, HEK-293 cells, U2OS cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85791-4-RR targets FAM134A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 FAM134A fusion protein Ag20025 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 family with sequence similarity 134, member A
别名 C2orf17, MAG2, Protein FAM134A, Reticulophagy regulator 2, RETREG2
计算分子量 543 aa, 58 kDa
观测分子量70-80 kDa
GenBank蛋白编号BC002420
基因名称 FAM134A
Gene ID (NCBI) 79137
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8NC44
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The protein family with sequence similarity 134 (FAM134) consists of three proteins, FAM134B, FAM134A, and FAM134C, which share the same LIR amino acid sequence. FAM134 reticulon family proteins (FAM134A, FAM134B, and FAM134C) are ER-phagy receptors with structural similarities and nonredundant functions. The protein family with sequence similarity 134 (FAM134). Fam134A (also known as metastasis-associated genes 2, MAG-2) is widely expressed in various types of tissues including tumors, and is considered to be closely correlated with tumor proliferation, invasion, and metastasis.

实验方案

Product Specific Protocols
WB protocol for FAM134A antibody 85791-4-RRDownload protocol
IHC protocol for FAM134A antibody 85791-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols