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验证数据展示

  • WB analysis using 85790-4-RR

    WB analysis using 85790-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85790-4-RR (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85790-4-RR

    Affinity and Kinetic Characterization of 85790-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85790-4-RR against Human Ezrin were performed. The affinity constant is 0.426 nM.

Ezrin Recombinant antibody

Ezrin Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85790-4-RR

产品说明书

CloneNo. 250065E1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

EZR, VIL2, Villin-2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Calu-3 cells, A431 cells, Jurkat cells, MOLT-4 cells, NIH/3T3 cells, C6 cells
Planning an IHC experiment? We recommend our IHCeasy Ezrin Ready-To-Use IHC Kit. Ezrin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85790-4-RR targets Ezrin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Ezrin fusion protein Ag23530 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ezrin
别名 EZR, VIL2, Villin-2
计算分子量 69 kDa
观测分子量70-80 kDa
GenBank蛋白编号BC013903
基因名称 Ezrin
Gene ID (NCBI) 7430
ENSEMBL Gene IDENSG00000092820
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP15311
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ezrin is a member of the ERM (Ezrin, Radixin, Moesin) protein family and serves as a crucial linker between the cell membrane and the actin cytoskeleton. It plays significant roles in various cellular processes, including maintaining cell morphology, facilitating cell movement and adhesion, regulating signal transduction, and influencing apoptosis.

实验方案

Product Specific Protocols
WB protocol for Ezrin antibody 85790-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols