验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, Jurkat cells, MCF-7 cells, U2OS cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85786-1-RR targets PLCB3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | phospholipase C, beta 3 (phosphatidylinositol-specific) |
| 别名 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3, EC:3.1.4.11, Phosphoinositide phospholipase C-beta-3, Phospholipase C beta 3, Phospholipase C-beta-3 |
| 计算分子量 | 1234 aa, 139 kDa |
| 观测分子量 | 139-150 kDa |
| GenBank蛋白编号 | BC142681 |
| 基因名称 | PLCB3 |
| Gene ID (NCBI) | 5331 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q01970 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLCB3 is a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Six subfamilies of PLCs (B, G, D, E, Z and H) constitute part of ubiquitous signaling cascades that translate hormonal signals into intracellular events, leading to alternations in cell function. PLCB isoforms 1-4 are stimulated by G-protein activation (Gαq/11 and/or Gβγ). Independent of its enzymatic activity, PLCB3 inhibits the proliferation of hematopoietic stem cells (HSCs) and myeloid cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLCB3 antibody 85786-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |