验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue, mouse cerebellum tissue, rat cerebellum |
Planning an IHC experiment? We recommend our IHCeasy SLC12A5 Ready-To-Use IHC Kit. SLC12A5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85751-1-RR targets KCC2/SLC12A5-Specific in WB, ELISA applications and shows reactivity with human, mouse, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, pig |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | solute carrier family 12 (potassium-chloride transporter), member 5 |
| 别名 | KCC2, KCC2/SLC12A5, SLC12A5, Electroneutral potassium-chloride cotransporter 2, hKCC2 |
| 计算分子量 | 126 kDa |
| 观测分子量 | 130-150 kDa |
| GenBank蛋白编号 | NM_020708 |
| 基因名称 | KCC2 |
| Gene ID (NCBI) | 57468 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9H2X9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KCC2, encoded by SLC12A5, is a neuron-specific K+-Cl- cotransporter that is essential for Cl- homeostasis and inhibitory synaptic transmission in brain. KCC2 is expressed at high levels in neurons throughout the nervous system. KCC2 deletion caused animal death. Alterations of KCC2 have been linked to neuropathic pain, temporal lobe epilepsy, and other diseases. This antibody can detect 130-150 kDa monomer and ~250 kDa dimer of KKC2 in brain lysate. (PMID: 23401525)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KCC2/SLC12A5-Specific antibody 85751-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
