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验证数据展示

  • WB analysis using 85717-1-RR

    WB analysis using 85717-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85717-1-RR (CYP2C8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 85717-1-RR

    IHC staining of mouse liver using 85717-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85717-1-RR (CYP2C8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 85717-1-RR

    IHC staining of mouse liver using 85717-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85717-1-RR (CYP2C8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CYP2C8 Recombinant antibody

CYP2C8 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85717-1-RR

产品说明书

CloneNo. 242948A3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CP2C8, 242948A3, CPC8, CYP2C8/9/18/19, CYPIIC8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85717-1-RR targets CYP2C8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CYP2C8 fusion protein Ag9898 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome P450, family 2, subfamily C, polypeptide 8
别名 CP2C8, 242948A3, CPC8, CYP2C8/9/18/19, CYPIIC8
计算分子量 490 aa, 56 kDa
观测分子量 50 kDa
GenBank蛋白编号BC020596
基因名称 CYP2C8
Gene ID (NCBI) 1558
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP10632
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYP2C8(Cytochrome P450 2C8) is also named as CYPIIC8, cytochrome P450 IIC2, cytochrome P450 MP-12, cytochrome P450 MP-20, cytochrome P450 form 1 and S-mephenytoin 4-hydroxylase. It belongs to the cytochrome P450 family. It is a monooxygenase involved in an NADPH-dependent electron transport pathway and a hepatic drug-metabolizing enzymes that oxidizes therapeutic drugs such as cerivastatin and endobiotics such as retinoic acid and arachidonic acid. It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for CYP2C8 antibody 85717-1-RRDownload protocol
IHC protocol for CYP2C8 antibody 85717-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols