验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293T cells, T-47D cells, HL-60 cells, Jurkat cells, Raji cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
85707-1-RR targets KIF14 in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human |
免疫原 | KIF14 fusion protein Ag23219 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | kinesin family member 14 |
别名 | Kinesin-like protein KIF14 |
观测分子量 | 186 kDa |
GenBank蛋白编号 | BC113742 |
基因名称 | KIF14 |
Gene ID (NCBI) | 9928 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q15058 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIF14 (Kinesin Family Member 14) is a microtubule-based motor protein belonging to the kinesin-3 family. It plays essential roles during cell division and has been implicated in various biological processes, including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. KIF14 is highly conserved across species and is characterized by a catalytic motor domain that binds to microtubules and ATP, enabling it to convert chemical energy into mechanical work. KIF14 interacts with the Skp1/Cul1/F-box (SCF) complex, particularly Skp2 and Cks1, to regulate the ubiquitination and degradation of p27Kip1. Knockdown of KIF14 results in the accumulation of p27Kip1, leading to cell cycle arrest and apoptosis.(PMID: 34131133, PMID: 24854087)
实验方案
Product Specific Protocols | |
---|---|
WB protocol for KIF14 antibody 85707-1-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |