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验证数据展示

  • WB analysis using 85686-1-RR

    WB analysis using 85686-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85686-1-RR (DDX6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 85686-1-RR

    IHC staining of mouse testis using 85686-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85686-1-RR (DDX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 85686-1-RR

    IHC staining of mouse testis using 85686-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85686-1-RR (DDX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DDX6 Recombinant antibody

DDX6 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85686-1-RR

产品说明书

CloneNo. 242957B2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ATP-dependent RNA helicase p54, EC:3.6.4.13, HLR2, P54, Probable ATP-dependent RNA helicase DDX6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, C2C12 cells, PC-12 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85686-1-RR targets DDX6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DDX6 fusion protein Ag6211 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
别名 ATP-dependent RNA helicase p54, EC:3.6.4.13, HLR2, P54, Probable ATP-dependent RNA helicase DDX6
计算分子量 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC065007
基因名称 DDX6
Gene ID (NCBI) 1656
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP26196
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX6, also named as P54, HLR2, and RCK, is a 483 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and belongs to the DEAD box helicase family. DDX6/DHH1 subfamily. P54 is abundantly expressed in most tissues. In the process of mRNA degradation, P54 may play a role in mRNA decapping. P54 is involved in the replication of hepatitis C virus genomes in hepatocytes and in tumourigenesis of hepatocellular carcinomas.

实验方案

Product Specific Protocols
WB protocol for DDX6 antibody 85686-1-RRDownload protocol
IHC protocol for DDX6 antibody 85686-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols