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验证数据展示

  • WB analysis using 85636-2-RR

    WB analysis using 85636-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85636-2-RR (CDO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat liver using 85636-2-RR

    WB analysis of rat liver using 85636-2-RR

    rat liver tissue were subjected to SDS PAGE followed by western blot with 85636-2-RR (CDO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

CDO1 Recombinant monoclonal antibody

CDO1 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 85636-2-RR

产品说明书

CloneNo. 250039D9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

CDO, CDO I, CDO-I, Cysteine dioxygenase type 1, Cysteine dioxygenase type I

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat liver tissue, mouse liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85636-2-RR targets CDO1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3294

Product name: Recombinant human CDO1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-200 aa of BC024241

Sequence: MEQTEVLKPRTLADLIRILHQLFAGDEVNVEEVQAIMEAYESDPTEWAMYAKFDQYRYTRNLVDQGNGKFNLMILCWGEGHGSSIHDHTNSHCFLKMLQGNLKETLFAWPDKKSNEMVKKSERVLRENQCAYINDSVGLHRVENISHTEPAVSLHLYSPPFDTCHAFDQRTGHKNKVTMTFHSKFGIRTPNATSGSLENN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cysteine dioxygenase, type I
别名 CDO, CDO I, CDO-I, Cysteine dioxygenase type 1, Cysteine dioxygenase type I
计算分子量 200 aa, 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC024241
基因名称 CDO1
Gene ID (NCBI) 1036
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16878
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDO1(cysteine dioxygenase type 1) is also named as CDO and belongs to the cysteine dioxygenase family. It is an enzyme that adds molecular oxygen to the sulfur of cysteine, converting the thiol to a sulfinic acid known as cysteinesulfinic acid (3-sulfinoalanine) and CDO1 is one of the most highly regulated metabolic enzymes responding to diet(PMID:19011731). The expression of CDO can significantly decrease the level of intracellular cysteine and this reduction is also associated with a decrease in total glutathione levels(PMID:17327371). Cysteine dioxygenase (CDO) plays a critical role in the regulation of cellular cysteine concentration andmultiple forms of CDO (23 kDa, 25 kDa, and 68 kDa) have been claimed based upon separation and detection using SDS-PAGE/western blotting(PMID: 14752623).

实验方案

Product Specific Protocols
WB protocol for CDO1 antibody 85636-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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