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验证数据展示

  • WB analysis of human placenta using 85446-2-RR

    WB analysis of human placenta using 85446-2-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 85446-2-RR (HPGDS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85446-2-RR

    Affinity and Kinetic Characterization of 85446-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85446-2-RR against Human HPGDS were performed. The affinity constant is 0.168 nM.

HPGDS Recombinant antibody

HPGDS Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85446-2-RR

产品说明书

CloneNo. 242980B8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

EC:2.5.1.18, EC:5.3.99.2, Glutathione S transferase, Glutathione S-transferase, Glutathione-dependent PGD synthase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85446-2-RR targets HPGDS in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 HPGDS fusion protein Ag18290 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 prostaglandin D2 synthase, hematopoietic
别名 EC:2.5.1.18, EC:5.3.99.2, Glutathione S transferase, Glutathione S-transferase, Glutathione-dependent PGD synthase
计算分子量 199 aa, 23 kDa
观测分子量26 kDa, 52 kDa
GenBank蛋白编号BC020734
基因名称 HPGDS
Gene ID (NCBI) 27306
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO60760
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hematopoietic PGD synthase (HPGDS), one of the PGD2 synthases, is distributed in the cytoplasm of mast cells and Th2 cells and catalyzes PGD2 production during inflammation and allergic reactions. HPGDS belongs to the gene family of σ-type glutathione S-transferase (GST). Like other GSTs, it exists as a homodimer in vivo with a molecular weight of approximately 52 kDa (PMID: 38339125).

实验方案

Product Specific Protocols
WB protocol for HPGDS antibody 85446-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols