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验证数据展示

  • WB analysis using 85426-2-RR

    WB analysis using 85426-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85426-2-RR (ALDH1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 85426-2-RR

    IHC staining of mouse testis using 85426-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85426-2-RR (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALDH1B1 antibody included
  • IHC staining of mouse testis using 85426-2-RR

    IHC staining of mouse testis using 85426-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85426-2-RR (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALDH1B1 antibody included
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ALDH1B1 Recombinant monoclonal antibody

ALDH1B1 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85426-2-RR

产品说明书

CloneNo. 242944E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Aldehyde dehydrogenase 5, Aldehyde dehydrogenase X, mitochondrial, ALDH5, ALDHX, EC:1.2.1.3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ALDH1B1 Ready-To-Use IHC Kit. ALDH1B1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85426-2-RR targets ALDH1B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7144

Product name: Recombinant human ALDH1B1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 168-517 aa of BC001619

Sequence: FCFTRHEPVGVCGQIIPWNFPLVMQGWKLAPALATGNTVVMKVAEQTPLSALYLASLIKEAGFPPGVVNIITGYGPTAGAAIAQHMDVDKVAFTGSTEVGHLIQKAAGDSNLKRVTLELGGKSPSIVLADADMEHAVEQCHEALFFNMGQCCCAGSRTFVEESIYNEFLERTVEKAKQRKVGNPFELDTQQGPQVDKEQFERVLGYIQLGQKEGAKLLCGGERFGERGFFIKPTVFGGVQDDMRIAKEEIFGPVQPLFKFKKIEEVVERANNTRYGLAAAVFTRDLDKAMYFTQALQAGTVWVNTYNIVTCHTPFGGFKESGNGRELGEDGLKAYTEVKTVTIKVPQKNS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aldehyde dehydrogenase 1 family, member B1
别名 Aldehyde dehydrogenase 5, Aldehyde dehydrogenase X, mitochondrial, ALDH5, ALDHX, EC:1.2.1.3
计算分子量 57 kDa
观测分子量50-55 kDa
GenBank蛋白编号BC001619
基因名称 ALDH1B1
Gene ID (NCBI) 219
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP30837
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112).

实验方案

Product Specific Protocols
WB protocol for ALDH1B1 antibody 85426-2-RRDownload protocol
IHC protocol for ALDH1B1 antibody 85426-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols