验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, MDA-MB-231 cells, U-87 MG cells |
| Positive IHC detected in | human tonsillitis tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85229-23-RR targets PD-L1/CD274 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD274 molecule |
| 别名 | CD274, PD-L1, B7 H1, B7-H1, hPD-L1 |
| 计算分子量 | 290 aa, 33 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC074984 |
| 基因名称 | PD-L1 |
| Gene ID (NCBI) | 29126 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NZQ7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PD-L1/CD274 antibody 85229-23-RR | Download protocol |
| WB protocol for PD-L1/CD274 antibody 85229-23-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
