验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, Raji cells, L02 cells, C2C12 cells, COS-7 cells, Vero cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85212-2-RR targets TRX2/TXN2 in WB, ELISA applications and shows reactivity with human, mouse, monkey samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, monkey |
| 免疫原 | TRX2/TXN2 fusion protein Ag3640 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | thioredoxin 2 |
| 别名 | TRX2, MT TRX, MTRX, Mt-Trx, Thioredoxin 2 |
| 计算分子量 | 166 aa, 18 kDa |
| 观测分子量 | 12 kDa |
| GenBank蛋白编号 | BC013726 |
| 基因名称 | Thioredoxin 2 |
| Gene ID (NCBI) | 25828 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q99757 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TXN2 (Thioredoxin-2) is also named as TRX2, MTRX, Mt-TRX, and regulates the mitochondrial redox state and plays an important role in cell proliferation. It has been shown that cells conditionally deficient in Trx2 undergo apoptosis in the absence of exogenous stress, accompanied by an accumulation of intracellular ROS, the activation of caspase 9 and caspase 3, and the release of cytochrome c into the cytosol. (PMID: 18441302).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRX2/TXN2 antibody 85212-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |